Fig. 3
From: Lineage dynamics of murine pancreatic development at single-cell resolution
Mesothelial cells are dynamic over developmental time and are predicted to give rise to vascular smooth muscle populations. a t-SNE visualization of merged mesenchymal clusters from E12.5 (n = 18 pancreata), E14.5 (n = 14 pancreata for batch 1; n = 11 for batch 2), and E17.5 (n = 8 pancreata) tissue. Mesenchymal clusters were identified at each timepoint, subclustered, merged together, and reanalyzed. Cells are colored by cluster or timepoint. Dotted circle highlights timepoint-segregated mesothelial clusters. b Dot plot of top differentially expressed genes in timepoint-specific mesothelial clusters (clusters 1, 11, and 17). Size of the dot represents proportion of the population that expresses each specified marker. Color indicates level of expression. c ISH for Pitx2 and Msln in E12.5 and E17.5 pancreata. Pitx2 expression was detected in E12.5, but not E17.5 mesothelium, whereas Msln was detected in E17.5, but not E12.5 mesothelium. Vimentin (Vim) IF staining depicts pancreatic mesenchyme. Dotted line indicates tissue boundary. Yellow arrows identify Pitx2+ mesothelial cells. Red arrows identify Msln+ mesothelial cells. Scale bar represents 50 µm. d Expression levels of VSM-related genes in merged mesenchymal clusters. Color intensity indicates level of expression. e Pseudotime ordering of mesothelial and VSM-related merged mesenchymal clusters. Colors correspond to t-SNE in a. All clusters are individually plotted in Supplementary Fig. 3j. f Cluster proportions over pseudotime. Pseudotime was binned into ten groups and the proportion of each cluster within that bin of pseudotime was calculated. g Model of lineage relationships among mesothelial and VSM-related mesenchymal populations based on pseudotime ordering in e
