Fig. 7

TSP1 is downregulated by ADT, and repressed in NEPC cells and xenografts models. a Changes of TSP1 protein expression and NE markers in AR-positive LNCaP cells treated with 10 μM MDV3100 without or with 5 nM androgen DHT for 72 h. b RT-qPCR of TSP1 expression in LNCaP cells treated with 10 μM MDV3100 without or with 5 nM androgen DHT for 48 h. c Differences in TSP1 protein expression between LNCaP cells and NEPC cells NE1.3 and 144-13. d Mining an independent study (GSE8702) for expression of TSP1 and NE markers after culturing LNCaP cells in CSS media for different lengths of time. e Expression patterns of TSP1 and NE markers obtained from transcript profiles of LNCaP xenografts⁴⁴. 16D: xenograft resistant to castration (CRPC). 42D: xenograft resistant to castration and then became resistant to enzalutamide treatment with NE phenotypes. f Examining expression patterns of TSP1, EZH2, and NE markers in prostate cancer PDX transcript profiles⁴⁵ (European Nucleotide Archive access #: PRJEB9660). Prostate adenocarcinoma PDX tumors LTL331 relapse after castration and become NEPC PDX tumors LTL331R⁴⁵