Fig. 8
Correlation between M2 macrophage marker, USP24, p300, NF-κB, β-TrCP, and DNMT1 in lung cancer specimens. a Eight weeks old of EGFRL858R transgenic mice were treated with doxycycline for 3 months or 5 months to induce the formation of lung cancer, and then sacrificed at indicated time points. Lungs were harvested and immunohistochemistry was performed to analyze the expression of indicated proteins. YM-1 positive area was marked with blue dashed lines. Scale bar represents 50 μm. b Immunohistochemistry staining was used to analyze indicated protein in 50 human lung cancer specimens. Each slide was given with different scores to indicate the intensity of protein signals. Samples with score 1 and 2 were considered as low protein expression, and score 3 and 4 were considered as high protein expression. Scale bar represents 50 μm. In slides stained with anti-USP24 antibody, 30 samples were identified as low expression, and 20 samples were identified as high expression. Expression level of indicated proteins in low USP24 expression groups or high USP24 expression groups were shown in percentage. Correlation between USP24 expression and p300, β-TrCP, and DNMT1 were examined by Fisher’s exact test. P value of the correlation of USP24 to p300, USP24 to β-TrCP, and USP24 to DNMT1 were 0.1825, 0.0002, and 0.0421, respectively
