Fig. 3
From: Roquin targets mRNAs in a 3′-UTR-specific manner by different modes of regulation
Roquin interacts with the Nfkbid 3′-UTR via multiple stem-loop structures. a Consensus secondary structure prediction of the minimal response element of the Nfkbid 3′-UTR (1–263) by mLocARNA. Consensus sequences of SL1 (residues 19–36), SL2 (residues 37–50), SL3 (residues 112–132), SL4 (residues 161–175), SL5 (residues 214–230), and SL6 (residues 243–257) are represented. The color annotation is described in Fig.1b and Supplementary Fig. 3a. b Schematic representation of the six conserved SL structures within the 3′-UTR of mouse Nfkbid. Wild-type loop structures (gray) of the six SLs were modified by individual loop mutations (LM: change of purine and pyrimidine bases, green). c, d Flow cytometry analysis of GFP in Rc3h1–2fl/fl; Cre-ERT2 MEF cells retrovirally transduced with the ICOS–GFP-Nfkbid 3′-UTR (1–263) reporter without mutation, with individual LMs (c) or with combined LMs and stem mutation in SL6 (SL6 SM) (d). MEF cells were either treated with 4′ OH-tamoxifen (iDKO) or left untreated (WT). Median fluorescent intensities (MFI) of GFP for WT (red) and iDKO (blue) MEF cells are indicated. Data are representative of six (c) and three (d) independent experiments
