Fig. 5 | Nature Communications

Fig. 5

From: Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import

Fig. 5

Physicochemical properties of TRAP clients with SP. The color coding follows Fig. 1. We used custom scripts to compute the hydrophobicity score (a) and glycine/proline (GP) content (b) of SP sequences. Hydrophobicity score was calculated as the averaged hydrophobicity of its amino acids according to the well-known Kyte-Doolittle propensity scale. GP content was calculated as the total fraction of glycine and proline in the respective sequence. Additional plots are shown in Supplementary Fig. 4a, b. We also used custom scripts to extract protein annotations for all human proteins (c) and yeast SP (d) from UniProtKB entries. TRAP homologs refer to yeast orthologs of eight TRAP dependent human proteins as indicated in Table 1. d Notably, the first peak refers to no GP per yeast SP, the second to one GP, the third to two GPs, and so on. This oscillatory appearance appears to be obscured by the higher variation in length of human SP

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