Fig. 7
INTERFACE can distinguish sRNA accessibility changes attributed to presence of a pleiotrophic RNA regulator. a INTERFACE is sensitive to Hfq dependency, as shown by changes in accessibility landscapes in a strongly Hfq-dependent sRNA (Supplementary Data 4) Spot 42 between the parent strain (+Hfq) and an isogenic Δhfq strain (−Hfq). Accessibility differences significant to P-value <0.1 in a paired two-tailed t-test are indicated by asterisks. b In contrast, no significant changes are observed for RybA, an sRNA believed to be Hfq-independent due to its inability to co-immunoprecipitate with Hfq in prior studies (Supplementary Data 4). The level of accessibility per region is indicated according to color (blue = low, gray = mid, red = high). c INTERFACE supports an accessibility-increasing role of Hfq in sRNAs, indicated by the skew of accessibility difference upon presence of Hfq relative to the absence of Hfq (parent – Δhfq) to the right for all significant differences (P-value <0.05, two-tailed t-test) within the entire sRNA ensemble (106 regions)
