Fig. 2

Effects of Nlg1 mutants on excitatory synapse differentiation. a Dendrites from DIV 14–15 cultured neurons co-transfected with GFP (green) and either EV, Nlg1-WT, -Y782A, or -Y782F. Neurons were live stained with an N-terminal GluA1 antibody (red) and counterstained with a VGlut1 antibody (blue). Scale bar, 5 µm. b, c Density of VGlut1 or GluA1 clusters, respectively, normalized to the EV condition (number of cells indicated within the bars, from 3 independent experiments). d Representative traces of mEPSCs recordings from DIV 14–15 neurons expressing EV, Nlg1-WT, -Y782A, or -Y782F clamped at −70 mV in the presence of TTX and bicuculline. e Cumulative distributions of the mEPSCs inter-event intervals for the corresponding conditions. f, g Mean mEPSC frequencies and amplitudes, respectively, for each condition (number of cells indicated within the bars, from 3 independent experiments). Data in graphs b, c, f, and g represent mean ± SEM and were compared by a Kruskal–Wallis test followed by Dunn’s multiple comparison test (ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001)