Fig. 5
From: Tracing the transitions from pluripotency to germ cell fate with CRISPR screening

Compound mis-regulation of WNT and germ cell genes in the absence of Nr5a2 and Zfp296. a Heatmap showing change in mean expression profile of selected genes from triplicate independent WT, Nr5a2−/− or Zfp296−/− lines during PGCLC induction by RNA-seq. b Quantitative validation of gene expression changes in PGCLC from WT, Nr5a2−/− and Zfp296−/− lines by independent qRT-PCR experiments. Error bars show s.e.m. of duplicate biological experiments, each comprising at least twelve independent replicate inductions. ND not detected