Fig. 6
From: Tracing the transitions from pluripotency to germ cell fate with CRISPR screening
Role of WNT and rescue of Nr5a2 defects. a Global levels of DNA methylation reprogramming in WT and mutant PGCLC assayed by LUMA. b qRT-PCR analysis showing expression of WNT genes/targets are affected by WNT inhibitor (+XAV939) or agonist (+chiron), similarly to knockout PGCLC. w/o: control without WNT modulation. c Expression of Nanog and mesendoderm gene Hey1 by qRT-PCR in WT and mutant PGCLC with WNT modulation. d Effects of WNT manipulation on efficiency of PGCLC ontogeny in quadruplicate independent experiments. Bar indicates mean value and *p < 0.05. e FACS plot showing the Nr5a2-mutant PGCLC specification defect at d2 is rescued by Dox-inducible activation of exogenous Nr5a2 cDNA. Dox is added after EpiLC induction. f Percentage Stella+ PGCLC induced without cytokines from WT cells upon DOX-inducible expression of the indicated cDNA
