Fig. 4
From: A variant NuRD complex containing PWWP2A/B excludes MBD2/3 to regulate transcription at active genes
PWWP2A and PWWP2B are enriched at actively transcribed genes. a PWWP2A binding patterns are highly correlated with H3K36me3 at high, intermediate, and low expressed genes (from 5 kb upstream of TSS to 5 kb downstream of TTS). Solid line: PWWP2A; Dash line: H3K36me3. The x-axis denotes the normalized genomic regions and average coverage signals for each gene group, respectively. The left and right y-axis represent the signal of PWWP2A and H3K36me3, respectively. b Scatterplot showing the correlation of gene body occupancy by PWWP2A and H3K36me3. c Percentage of PWWP2A, PWWP2B, HDAC1, MTA2, MBD3, and CHD4 occupied genes by expression level (H High, I Intermediate, L Low, N No). The majority of PWWP2A and PWWP2B bound genes are highly expressed genes. d Heatmaps showing the binding profile across all gene loci for PWWP2A, H3K36me3, PWWP2B, HDAC1, MTA2, MBD3, CHD4 sorted by occupancy of PWWP2A from 5 kb upstream of the TSS to 5 kb downstream of the TTS. H3K4me3 is shown from −5 kb to +5 kb of the TSS. e A representative example of UCSC genome browser tracks displaying the ChIP-seq profile at the Mta2-Tut1 locus. The long protein isoform of PWWP2A co-localises with H3K36me3. The short isoform PWWP2ASI and PWWP2A∆PWWP have no detectable gene body binding. The y-axis represents the normalized coverage
