Fig. 1

The ZZ domain of ZZZ3 recognizes histone H3 tail. a Schematic representation of ZZZ3 protein structure. The amino acid numbers of ZZ domain and the full-length protein are shown. b Histone peptide microarray probed with GST-ZZZ3 ZZ domain. Each peptide has three replicates. c Western blot analysis of GST pulldowns of GST-ZZZ3 ZZ with calf thymus histones. Pulldown of GST-RBP2 PHD1 is shown as a control. d Western blot analysis of peptide pull-down experiments of GST-ZZZ3 ZZ domain with the indicated histone H3 peptides. Peptide pulldowns of RBP2 PHD1 and WDR5 are shown for comparison. e–g Western blot analysis of peptide pulldown experiments of GST-ZZZ3 ZZ domain with the indicated H3 peptides. Peptide pulldowns of WDR5 are shown for comparison. h Phylogenetic tree presentation of the H3-binding activity of human ZZ proteins. The red dot indicates binding with H3 (Supplementary Fig. 1c–e). The cytoplasmic ZZ proteins (gray) are not tested in this study