Fig. 2
Structure of the ZZZ3 ZZ domain in complex with H3 peptides. a Ribbon diagram of the ZZZ3 ZZ816–874 domain (green) in complex with the histone H3 peptide (yellow). b Electrostatic potential surface of ZZZ3 ZZ in complex with the histone H3 peptide (yellow stick). A positive electrostatic potential is colored in blue and negative in red. c Zoom-in view of the histone H3 Ala1 binding site. ZZZ3 ZZ domain is shown as a green ribbon and the histone H3 peptide as a yellow stick. The dash lines indicate short distances. d Binding affinities of WT ZZZ3 ZZ and mutants for the indicated histone peptides measured by tryptophan fluorescence. The experiments were carried out in triplicates. *: measured by NMR. e Binding curves used to determine the Kd values by MST. f Zoom-in view of the mean position of Lys4 in each ensemble of 20 NMR structures of the complexes of ZZZ3 ZZ with unmodified H3 peptide and with H3K4ac peptide are superimposed. g Western blot analysis of histone peptide pulldowns of wild-type (WT) ZZZ3 ZZ and the indicated point mutants
