Fig. 1
CB1 and PAX7 gene expression in dystrophic skeletal muscles and cells. a, b Time course of CB1 and PAX7 mRNA expression levels in gastrocnemius (a) and quadriceps (b) muscle of control (dark yellow columns) and mdx (red columns) mice of 3 (n = 8), 5 (n = 8), and 8 (n = 8) weeks of age. The quantification of transcripts for CB1 and PAX7 was performed by quantitative real-time PCR. c Heatmap representation of selected genes obtained from RNA-seq analysis in fibroadipogenic (FAP), satellite (SC), and macrophage (MP) cells isolated from 8-week-old control (n = 4) and mdx (n = 4) mice. Red, upregulated; green, downregulated. d Bar graph showing RPKM (Reads Per Kilobase of transcript per Million mapped reads) normalized values for the CB1 gene obtained from RNA-seq analysis in isolated FAP, SC, and MP cells. Each bar is the mean ± SEM of the independent determinations. *P ≤ 0.05 vs. control animals or cells, determined by Student’s t test
