Fig. 2

CB1 and PAX7 expression and 2-AG levels in dystrophic muscles. a mRNA expression levels of CB1 and PAX7 in muscle samples of healthy children (HT, controls n = 3) or DMD donors of 3 (n = 4) and 7 (n = 4) years. b Measurement of the endogenous levels of 2-AG in muscle samples of the same healthy and DMD donors. Time course of the endogenous levels of 2-AG in the quadriceps (c) and gastrocnemius (d) muscle of control (dark yellow; n = 8) and mdx (red columns; n = 8) mice. The levels of 2-AG are expressed as pmol mg⁻¹ of wet tissue weight. *P ≤ 0.05 vs. control group, determined by Student’s t test. e The bar graphs show RPKM (Reads Per Kilobase of transcript per Million mapped reads) normalized values for the Daglα and Magl genes obtained from RNA-seq analysis in satellite cells isolated from 8-week-old wt (n = 4) and mdx mice (n = 4)