Fig. 3
Bioinformatics and ChIP analysis. Schematic representation of the human (a) or murine (b) CB1 gene. The transcriptional start site (TSS) for each gene isoform is indicated. The identified PAX7 sites are shown as small black rectangles below the schematic. Regions identified as 1–4, indicated by red arrows, correspond to PAX7-containing gene regions of high sequence homology in human and murine CB1 gene. c PAX7 occupancy of identified sites in the CB1 gene evaluated by chromatin immunoprecipitation (ChIP) analysis. Left: average data of the relative amount of the PAX7-immunoprecipitated DNA in the murine quadriceps muscle isolated from either wild-type or mdx mice; Right: average data of the relative amount of the PAX7-immunoprecipitated DNA in PAX7-silenced satellite cells. Data are from six separate experiments and normalized relative to the input DNA. The inset shows a representative agarose gel electrophoresis of the qPCR products obtained from PAX7-immunoprecipitated DNA for each experimental condition. d Left: representative agarose gel electrophoresis of the qPCR products obtained from PAX7-immunoprecipitated DNA for each experimental condition. Right: average data of the relative amount of the PAX7-immunoprecipitated DNA in HEK293 cells transfected with control scramble (n = 4) human PAX7 construct (n = 4). *P ≤ 0.05 vs. control group, determined by Student’s t test
