Fig. 4
Luciferase assay in PAX7-transfected HEK293 cells. Relative luciferase activity following transfection of human constructs into HEK293 cells. Cells were co-transfected with the pGL3 promoter vector carrying the construct of interest (regions 2, 3, and 4) cloned upstream of the luciferase gene together with an equimolar amount of plasmid encoding for human PAX7. For the control condition, PAX7 plasmid was replaced with pCDNA3.1. Cells were co-transfected with a β-Gal encoding vector to normalize transfection efficiency and the signal intensity of luciferase. The inset on the right shows a representative chemiluminescent signal emitted from the reaction of luciferase from the different experimental conditions. Data are expressed as the mean ± SEM of four independent determinations. *P ≤ 0.05 vs. control group, determined by Student’s t test
