Fig. 9
Measurement DAGs levels and activity in satellite cells and muscles. Levels of diacylglycerols (DAGs) (a) and 2-AG (b), in primary satellite cells exposed to the selective CB1 agonist ACEA 3 µM for 20 min. The levels of 2-AG and DAGs are normalized to the amount of lipids extract (pmol mg−1). Each bar is the mean ± SEM of six separate determinations. *P ≤ 0.05 vs. vehicle group, determined by Student’s t test. c Representative blots showing the chemiluminescent signal generated by the anti-phosphospecific (Thr497 site) and non-phosphospecific PKC antibody in satellite cells exposed to ACEA 3 µM for 20 min or d in the gastrocnemius isolated from control (n = 4) and mdx (n = 4) mice at 8 weeks. Quantification of phospho-PKC (pPKC) levels are normalized to total PKC. Each bar is the mean ± S.E.M. of five separate determinations. *P ≤ 0.05 vs. the indicated experimental group, determined by Student’s t test
