Fig. 3

Worse cortex CBF and CC hypoxia in ApoE4-TR mice after BCAS. a Neocortical CBF and mean arterial pressure (MAP) recorded at the indicated times over 4 weeks before or after sham surgery (WT) or BCAS in WT (WT-Sham or -BCAS), ApoE3 (ApoE3-BCAS), and ApoE4 (ApoE4-BCAS) mice. Right and left CBF values were not different and were averaged. The CBF reduction induced by BCAS is more marked in ApoE4-TR mice. b Representative laser speckle images of CBF before or after BCAS in WT, ApoE3-TR, and ApoE4-TR mice, confirming the greater CBF reduction in ApoE4-TR mice (see Supplementary Fig. 7C for quantification). Scale bar = 2 mm. c The intensity of the hypoxyprobe signal, a marker of tissue hypoxia, is more pronounced in the CC of ApoE4-TR mice, compared to WT or ApoE3-TR mice. Data are expressed as means ± SEM. Scale bar = 250 µm. N = 5/group; * p < 0.05 from WT-sham; ^# p < 0.05 from WT and ApoE3-BCAS; one-way ANOVA and Tukey’s test. ALU arbitrary fluorescent intensity units