Fig. 5
From: Apoε4 disrupts neurovascular regulation and undermines white matter integrity and cognitive function
Worse CC injury and cognition in ApoE4-TR mice after BCAS. a Klüver-Barrera (KB) stain of the CC in sham-operated WT mice and in WT, ApoE3-TR, and ApoE4-TR mice after BCAS. In a, as in b, c, d, related quantification shown on the right. Scale bar = 250 µm. b Double-label immunohistochemistry with myelin basic protein (MBP) and the pan-axonal neurofilament marker SMI312 in the same groups of mice. Scale bar = 25 µm. c Myelin-associated glycoprotein (MAG) immunohistochemistry in the same groups of mice. ALU: arbitrary fluorescent intensity units. Scale bar = 250 µm. d Double-label immunohistochemistry for the nodal Nav1.6 channels and the paranodal protein Caspr in the same groups of mice. Scale bar = 1.5 µm. e, f ApoE4-TR mice have a more impaired arm alternation at the Y-maze test (e) and worse performance at the novel object recognition test (f). Data are expressed as means ± SEM. N = 5/group in a–d; N = 10/group in E–F; * p < 0.05 from WT-Sham; # p < 0.05 from WT- or ApoE3-BCAS; one-way ANOVA and Tukey’s test
