Fig. 1

Spontaneous recovery awaits successful repair. a Timing of cell cycle exit by onset of Cyclin B1 translocation (T) or spontaneous recovery by mitotic entry (M) after IR with indicated doses. Pooled cells from three independent exp. n > 20 Mean + sd. b Number of γH2AX foci in interphase (I) cells 1 h after IR and recovering (mitotic, M) cells collected 5–7 h after IR with indicated doses. Pooled cells from three independent exp. n > 65 + sd. c Stills representing RPE CCNB1^YFP cells that enter mitosis or that translocate Cyclin B1 after IR (2 Gy) and the corresponding 53BP1^mCherry signal in the nucleus. Scale bar 10 μm. d Quantification of 53BP1 foci from live cell imaging at indicated timepoints after IR in cells that recover spontaneously and cells that translocate Cyclin B1. n = number of cells pooled from three independent experiments + 95% confidence interval