Fig. 2

Lung cDC1s are necessary and sufficient for the induction of eosinophilia. a Lung cDC1s from WT and Batf3^−/− mice. b Eosinophil counts in the lung or Balf in WT (solid circle) and Batf3^−/− (empty circle) mice 1.5 days after the first OVA challenge. n = 4 or 6 mice (Saline) or n = 5–6 mice (OVA). c, d Mice were sensitized and challenged with papain, and eosinophils in the lung or Balf in WT (solid circle) and Batf3^−/− (empty circle) mice were analyzed. n = 5–7 mice per group. e Lung cDC1s from DT treatment langerin-DTR or WT mice. f, g OVA/alum-sensitized (f) or papain-sensitized (g) langerin-DTR (empty circle) or WT (solid circle) mice were treated with DT on day −1, and eosinophils in the lung or Balf were assessed after challenge. n = 5–9 mice per group. h, i Eosinophils recruited into the air pouches injected with pulmonary cDC1s (SiglecF⁻CD11c⁺IA/IE⁺CD103⁺CD11b⁻) or CD11b⁺ DCs (1.5 × 10⁴, 200 μl) sorted from C57BL/6 mice 1.5 days after the first OVA challenge (h) or 1 day after the first papain challenge (i). n = 4–6 mice per group. j Eosinophils recruited into the air pouches injected with pulmonary cDC1s, CD24⁺ cDC2s, CD24⁻CD11b⁺ DC2s, MCs, or IMs (1.5 × 10⁴, 200 μl) sorted from C57BL/6 mice 1.5 days after the first OVA challenge. n = 4–6 per group. k The direct chemotactic effect of lung cDC1s, CD24⁺ cDC2s, or MCs (from C57BL/6 mice 1.5 days after the first OVA challenge) on eosinophils was evaluated in a transwell culture system. n = 3–4 per group. l Protocol (left) and lung eosinophil counts (right) of allergic airway induction by adoptive i.t. injection of lung cDC1s, CD24⁺ cDC2s, or MCs; see Methods *P < 0.05, **P < 0.01, ***P < 0.001, unpaired Student’s t test. Means ± SD are shown. Data represent two (c, d, g, i, l) and three (a, b, e, f, h, j, k) independent experiments