Fig. 2
From: Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations
20 distinct cell populations were revealed in healthy human livers. a Viable cells were identified from the single-cell libraries having a minimum library size of 1500 transcripts and a maximum of 50% mitochondrial transcript proportion. b t-SNE projection of 8444 liver cells (each point represents a single cell). Cells are colored by library size, with darker colors indicating larger libraries. c t-SNE projection where cells that share similar transcriptome profiles are grouped by colors representing unsupervised clustering results. d Heat map analysis using known gene expression profiles of hepatocytes/immune cells. The identity of each cluster was assigned by matching the cluster expression profile with established cell-specific marker gene expression for hepatocytes, endothelial cells, cholangiocytes, and immune cells. e Cell-cycle phase prediction showed that hepatocyte clusters were less proliferative than immune cell clusters. f Cluster map showing the assigned identity for each cluster defined in c. The cluster number of each potential cell population is indicated in parentheses. DE: differentially expressed, MACs: macrophages, PCA: principal component analysis, t-SNE: t-distributed stochastic neighbor embedding, PCs: principal components
