Fig. 2

LC development in newborn mice and following BM transfer requires RARα. a Kinetics of langerin up-regulation on CD11c⁺ MHC-II⁺ cells in the epidermis of WT and ∆Rara^CD11c mice determined at postnatal day 1, 3, and 9. b Phenotype of epidermal DCs in new born (1-day old) mice. Trunk skin of WT and ∆Rara^CD11c mice was examined for CD11c⁺ MHC-II⁺ cells. c A BM reconstitution study from WT or ∆Rara^CD11c mice into lethally irradiated ∆Rara^CD11c mice. As WT control mice, we used either C57BL/6 or CD11C-Cre-GFP mice. The frequency of total CD11c⁺ MHC-II⁺ cells and epidermal langerin⁺ cells (% of CD11c⁺ MHC-II⁺) were examined 12–15 weeks post-BM transfer. Representative and combined data (n = 4–8) from at least 3 experiments are shown. *Significant differences from control mice by Mann–Whitney U test (p < 0.05, unpaired, 2-sided). Error bars are defined as s.e.m