Fig. 5
Mfn1 and Mfn2 play a minor role in CM maturation. a SRF bioChIP-seq signal showed proximal and distal chromatin binding near Mfn1 and Mfn2 genes specifically in actively maturing CMs. b Experimental design of mosaic, neonatal depletion of both MFN1 and MFN2. c Depletion of Mfn1 and Mfn2 mRNA (left) and protein (right) in FACS-sorted GFP/Dendra2+ CMs at P14. Mfn1 and Mfn2 mRNA expression was measured by RT-qPCR. MFN1 and MFN2 protein expression was measured by Western blotting. d FACS-EM analyses showed decreased mitochondria size upon Mfn1/2 depletion. e Effect of MFN1/2 depletion on mitochondrial organization. In situ imaging of TMRM staining. f Effect of MFN1/2 depletion on T-tubule morphology. In situ T-tubule imaging was quantified by AutoTT. g Effect of MFN1/2 depletion on sarcomere organization. h Effect of MFN1/2 depletion on cell size and geometry. Violin plots are described in Fig. 2. Non-significant P values are shown within parentheses. Bar plots show mean ± SD and are overlaid by dot plots. Scale bar, 20 μm. Two-tailed Student’s t test: *P < 0.05, ***P < 0.001
