Fig. 8

SRF overexpression perturbs the transcriptional program in maturing CMs. a SRF overexpression and control P14 CMs were FACS sorted and analyzed by RNA-seq. MA plots showed dramatic transcriptional dysregulation. Significantly dysregulated genes (Padj < 0.05) were dots in red. b Up-regulated genes upon SRF overexpression showed little overlap with genes down-regulated upon Srf KO at the same stage, as viewed by Venn diagram. c Gene ontology terms enriched among genes up-regulated upon SRF overexpression. The GSEA normalized enrichment scores for the top 5 terms were plotted. d Selected markers of endothelial cells (EC), smooth muscle cells (SMC), and fibroblasts (FB) that were up-regulated by SRF overexpression. e IPA upstream regulator analysis showed up-regulation of inflammatory response pathways upon SRF overexpression. The color of the lines signifies the expected direction of effect between two nodes. Blue represents predicted inhibition and orange represents predicted activation. Yellow signifies inconsistency between the gene expression in the data set and the annotated relationship. Gray indicates no prediction. Solid and dashed lines indicate direct and indirect interactions, respectively. f Gene ontology terms enriched among genes down-regulated upon SRF overexpression. g Statistical analyses of the differential expression of major sarcomere genes. Fold changes and P values were plotted in blue-yellow and magenta-green color scales, respectively. Differential expression analysis P values were calculated by DESeq2⁵⁹