Fig. 3
Age-affected pathways in the individual cell populations. a A selection of pathways from the Reactome database that show the most prominent changes upon ageing are depicted. Pathways were required to have between 5 and 150 members to be sufficiently covered in at least one cell population (>30% of the proteins are quantified), and to have at least 20% of its quantified components being significantly (p value < 0.05, Spearman's correlation) altered upon ageing. The figure illustrates the five most up- and down-regulated pathways for each cell population. The area of the bubbles represents the percentage of proteins quantified by TMT that are significantly (p value < 0.05, Spearman's correlation) altered. If no bubble is shown, no protein of the pathway was significantly altered in the respective cell population or no protein of the pathway has been quantified. Red indicates an overall increase of the pathway members with at least three proteins being up-regulated and pink indicates an overall increase with one or two proteins being up-regulated. Blue codes for pathways with an overall trend towards downregulation, with dark blue coding for pathways with at least three proteins being down-regulated and light blue containing one or two proteins being down-regulated. The colour white indicates that no overall tendency could be observed. The bars on the right-hand side of each pathway illustrate the number of proteins being significantly altered upon ageing regardless of the cell population (green), being quantified by TMT (grey), and the total number of members of the pathway (black) as also mentioned in the pathway annotation (n). The grouping of the pathways on the left side is based on the highest hierarchy levels defined in Reactome, e.g., extracellular matrix organization (ECM org.). b Effect of ageing on the abundance of DNMT1 and IRF8 in HPCs. The dots represent the individual results from young (age < 30 years) and old (age > 50 years) human subjects. The central line in the box plots indicates the median, the bottom and top edges of the box the interquartile range (IQR), and the box plot whiskers represent 1.5 times the IQR
