Fig. 6
Classification of single CD34+ cells into myeloid- and lymphoid-primed cells. a The matrices show the correlation (Pearson) of the abundance of the mRNA markers specific for lymphoid or myeloid differentiation (see also Fig. 5c) in single HPCs. b The distribution of correlation values (Pearson) between myeloid and lymphoid markers (grey), between myeloid markers only (pink), and between lymphoid markers only (purple) is presented as box plot. The central line in the box plots indicates the median, the bottom and top edges of the box the IQR, and the box plot whiskers represent 1.5 times the IQR. Correlation values are taken from all four samples. Significance was assessed using Mann–Whitney U-test (**p value < 0.01). c Cells are classified into lymphoid- and myeloid-primed cells based on markers. The bar-plot gives an overview on the fraction size of lymphoid-primed (purple), myeloid-primed (pink), and undefined (grey) cells in a given sample (undefined meaning that cells could not be classified as myeloid or lymphoid). For each fraction and each sample (S1–S4) the heat maps show the mRNA abundance of lymphoid and myeloid markers. The significance of marker distributions in each fraction was assessed by Fisher's exact test (p values depicted)
