Fig. 7
Single-cell analysis reveals lineage- and age-dependent increase of glycolytic enzymes. a The density plot shows distributions of correlation coefficients (Pearson) between lineage markers and glycolytic enzymes. The calculation is based on the results from the single-cell RNA-seq data. Density distributions based on correlations between myeloid (pink) or lymphoid (purple) markers with glycolytic enzymes that were up-regulated upon ageing, are shown with thick lines. The corresponding individual correlation values are displayed as lines in the box below the plot in the respective colours. Density distributions based on correlations of the respective markers with glycolytic and TCA-related proteins that did not change upon ageing are shown as dashed coloured lines. The grey distribution represents the correlation values of all proteins against myeloid and lymphoid markers. b Scatter plot illustrating the effect of ageing on glycolytic enzymes in lymphoid- and myeloid-primed cells, as deduced from single-cell analysis. The dots correspond to proteins in glycolysis that were not affected by age (grey), and glycolytic proteins that were altered upon ageing according to the proteomics data. The x-axis illustrates the relative abundance of those enzymes in myeloid-primed cells (left), and lymphoid-primed cells (right). The y-axis corresponds to the ageing slope derived as a measure of age-effect in lymphoid- and myeloid-primed cells across donors *p value < 0.05, Mann–Whitney U-test (see also Supplementary Fig. 12a, b). The data from the scatter plots are collapsed into box plots on both axes with the central mark indicating the median, the bottom and top edges of the box indicating the IQR. The box plot whiskers represent 1.5 times the IQR
