Fig. 3

Ectopic expression of c-Jun partially rescues the development of Med23^−/− iNKT cells. a Quantitative RT-PCR analysis of Med23 mRNA levels in WT thymic iNKT cells at stage 1, stage 2, and stage 3 as sorted by flow cytometry (n = 9). b The expression of representative genes with known transcriptional regulation between WT stage 2 and stage 3 iNKT cells (fold change ≥ 2 and p-value ≤ 0.05). c The expression of genes included in the AP-1 pathway in WT thymic iNKT cells at stage 2 and stage 3. Absolute expression values were transformed into Z scores before visualization. d c-Jun transcriptional levels in thymic iNKT cells at stage 2 and stage 3 from WT mice and stage 2 from Med23^−/− mice (n = 5). e The percentage of iNKT cells at stage 2 and stage 3 in the thymi of active c-Jun- or vector-transduced BM chimeric mice (WT-vector, n = 7; KO-vector, n = 6; WT-c-Jun, n = 6; KO-c-Jun, n = 5). f The percentage of iNKT cells at stage 2 and stage 3 in the livers of active c-Jun- or vector-transduced BM chimeric mice (WT-vector, n = 10; KO-vector, n = 7; WT-c-Jun, n = 9; KO-c-Jun, n = 6). g Quantitative RT-PCR analysis of representative genes with known regulation in the transition from stage 2 to stage 3 (Tbx21, Egr2, Med1, Tsc1, Pten) in sorted thymic stage 2 iNKT cells from WT mice and Med23^−/− mice (Tbx21, WT, n = 3, KO, n = 4; Egr2, n = 3; Med1 and Pten, n = 4; Tsc1, WT, n = 4, KO, n = 5). All expression levels (a, d, g) were normalized to Gapdh expression. The data are presented as the mean ± s.d. For all panels: *P < 0.05; **P < 0.01; ****P < 0.0001 by Student’s t-test; N.S. no significance. All data are representative of or combined from at least three independent experiments