Fig. 3

Lnk deficiency does not rescue ICL hypersensitivity associated with Fancd2 deficiency. a HSPCs were cultured in a graded concentration of MMC for 7 days. Total (right) and relative to untreated cell number (left) are shown. Statistical analysis using two-tailed Student’s t-test of comparisons among untreated and MMC-treated cells within each genotype are indicated. b–d Fresh BM cells were plated in semi-solid methylcellulose media containing various concentrations of MMC and assessed for progenitors of myeloid (b), B cell (c), and erythroid lineages (d). Relative colony forming units relative to untreated are shown. *p < 0.05, **p < 0.01, two-tailed Student’s t-test, denotes comparison between WT and D2; † p < 0.05, †† p < 0.01, denotes WT vs DKO, at the corresponding dose of MMC. Representative data from three independent repeats are shown