Fig. 5
Lnk deficiency rescues HSC defects associated with FA upon forced in vivo proliferation. Proliferation of HSPCs in vivo was induced by pI:pC injection. a depicts a schematic overview of pI:pC injection. Mice were injected i.p. with 5 mg/kg pI:pC twice per week over four weeks, followed by analysis for HSPC frequency and function in bone marrow at day 28. b, c LT-HSC frequency determined by SLAM LSK (b) or CD34-Flk2-SLAM LSK (c) marker schemes is shown. Bars indicate mean of frequencies from four to six mice, and error bars indicate SE. Statistics were calculated by two-tailed Student’s t-test. d Two million total BM cells from pI:pC-treated mice were mixed with 3 × 105 competitors and transplanted into lethally irradiated host animals. e In an independent experiment, one million total BM from pI:pC-treated mice were transplanted. d, e Donor chimerisms in peripheral blood of recipients 16 weeks after transplant are shown. Each symbol represents an individual recipient animal, horizontal bars represent the mean of each group. p values from two-tailed Student's t-test are shown. Two independent experiments are shown
