Fig. 4

Global gene expression and DNA methylation analysis. a Principal component analysis indicates that both PB-iNSCs and CB-iNSCs exhibit global gene expression profiles similar to those of their PSC-derived counterparts, but clearly distinct from their parental blood cells. b–e Scatter plot analysis of global gene expression profiles shows that PB-iNSCs are very distinct from PBCs (b). PB-iNSCs resemble lt-NES (c) and display a particularly high similarity to smNPCs (d). PB-iNSCs show a high degree of similarity with CB-iNSCs (e). Principal component analysis (a) and pair-wise scatterplots (b–e) were done genome-wide including 31,427 genes without filtration. f Heatmap of selected genes representative for neural, hematopoietic, and pluripotent cells reveals the expression of genes specific to the neural lineage in iNSCs. The clustering of different cell populations is based on the selected genes shown in the heatmap. g Hierarchical clustering of global DNAm profiles (485,512 CpGs) shows that the DNAm profiles of iNSCs are similar to those of PSC-derived NPCs and very distinct from PBCs and PSCs. The y-axis represents the height of dendrogram calculated using Euclidean distance and Ward's linkage method. PC: principle component; CBCs: CB-CD34⁺ cells; LP: low passages; HP: high passages. Triangles, diamonds, and squares indicate isogenic backgrounds