Fig. 6
Age-associated transcriptional signatures of iNSCs and PSC-derived NPCs. a Clustering of global transcriptional profiles of PBCs, iNSCs, and isogenic smNPCs at low (P ≤ 6) and high (P ≥ 18) passages. b Number of DEGs between different cell populations (q-value ≤ 0.05, absolute fold-change ≥ 1). c RNA seq data of post-mortem PFC tissues revealed 2,475 age-associated genes differentially expressed between young (<49 years of age) and old (≥49 years of age) brain samples. The Venn diagram shows that genes differentially expressed in low passage iNSCs vs. iPSC-derived low passage smNPCs include only 132 out of 2,475 genes (5.3%) differentially expressed in young vs. old PFC. d Scatter plot analysis of age-associated genes showing little difference between low passage iNSCs and smNPCs. e, f Expression level distribution of the 470 age-associated DEGs that are shared by all 3 comparisons (young vs. old PFC as well as low passage iNSCs and smNPCs vs. PBCs) shows no difference across low and high passage smNPCs and iNSCs. In contrast, gene expression level distribution across the young and old PFC samples shows a clear separation (Supplementary Fig. 12e, f) between the two groups. Data are shown for genes with higher expression levels in young (e) and higher expression levels in old (f) PFC samples. Box plots display the median (center line), the 25th and 75th percentile (upper and lower box, respectively) and 1.5-times the interquartile range from the box (whiskers). Data points outside this region are not shown
