Fig. 2
From: Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells
Loss of PKM2 leads to angiogenic sprouting defects. a EdU-positive cell numbers in control, PKM2KD and PKMKD ECs (n = 11). b Relative cell numbers at the indicated time points in control, PKM2KD and PKMKD ECs (n = 6). c Scratch wound assay in control and PKM2KD ECs (n = 8). d Representative confocal projections of 48 hpf Tg(kdrl:EGFP) pkma2+/+ pkmb+/+ and pkma2MZ pkmb−/− zebrafish embryos (scale bar = 50 μm). e Percentage of intersegmental vessels (ISVs) that have failed to connect with neighbouring ISVs in 48 hpf zebrafish embryos (n = 8). Data in a–c and e represent means ± s.e.m. (***P < 0.001 by one-way analysis of variance (ANOVA) followed by Tukey’s HSD test (a, e) or two-tailed Student's t test (c))
