Fig. 4
Mutagenesis of the residues located in the binding pockets of DmtB1. a Superposition of the binding pockets in the DmtB1 model with those in the crystal structure of YvmC. The P1 and P2 of the DmtB1 model are shown in cyan and green, respectively; the P1 and P2 of YvmC (PDB id: 3OQJ) are shown in wheat and brown, respectively. b HPLC traces of the products formation by DmtB1 and its variants (also see Supplementary Fig. 10). c Histogram of the relative formation of various DKPs synthesized by DmtB1 and its variants after 20 hrs of expression in E. coli at 16 °C. The corresponding western blots indicating amounts of proteins are also shown. Error bars represent ± s.d. of three independent experiments. d Total ion chromatogram in multiple reaction monitoring (MRM) mode for compounds 2–5 resulting from in vitro assays of DmtB1 and its variants (L185F and V205M). The relative titers of compounds were compared based on the peak area of MS2 fragment 130.11 from each molecular ion
