Fig. 8

Impacts of mutagenised DmtA1 on the final cyclization reaction. a Predicted DmtA1 model generated by utilizing TMRPres2D. b HPLC traces of ΔdmtA1 complemented with different dmtA1 variants (also see Supplementary Fig. 59). The cyclization products 9 and 12 were indicated in red. c Relative formation of DMT G (9) production in ΔdmtA1 complemented with different dmtA1 variants in comparison to complementation with wild-type dmtA1 (WT). Error bars represent ± s.d. of three independent experiments