Fig. 4
Development of convergent mutations within the Arg97 + disulfide sublineage. a Intron-only ML tree for all VRC26 lineage members. The Arg97 + disulfide clade is highlighted by number of shared intronic mutations with ADS-4. b The exon-only ML tree for all VRC26 members is shown with the location of the highlighted sequences in a indicated. c CDRH3 and intron alignment for the representative sequences marked in a and b. Three CDRH3 sites that eventually develop convergent mutations are circled and longitudinal time points of isolation are also indicated. ADS-4 intronic mutations are labeled and sequences are colored by the number of these mutations that they possess. d Number of Arg97 + disulfide clade sequences that share intronic mutations with ADS-4 and their amino acid composition at CDRH3 Kabat position 100n. e Same as in d except shown by longitudinal time point of isolation. f Same as d except shown for CDRH3 position 102. g Same as in e except for CDRH3 position 102. h Same as in d except for CDRH3 position 100o. i Same as in e except for CDRH3 position 100o
