Fig. 7
Late-stage bNAb intermediates and intraclonal convergence. a CDRH3 alignment of potential VRC26.19 intermediates (VRC26.19-I1, VRC26.19-I2), VRC26.19-like sequences (VRC26.19-(1–3)) and VRC26.09-like sequences (VRC26.09-(1–3)). VRC26.19-related sequences are highly diverse when compared between the two time points with about 70–76% CDRH3 amino acid identities between members of the two groups. CDRH3 sites containing residues that are candidates for intraclonal convergence throughout the lineage are marked in red. Sites with CDRH3-stabilizing mutations are marked in blue. b Alignment of the introns of the sequences shown in a. SHM events (point mutations or indels) that are shared between at least one member of each time point for VRC26.19-related sequences are marked with arrows and labeled according to germline positions. The same sites are marked for VRC26.09-like sequences for comparison. c Lower major bifurcating branch timeline with the potential intermediates for VRC26.19 indicated in bold. d List of sublineages and their candidate convergent CDRH3 mutations with the most prevalent codon shown
