Fig. 8 | Nature Communications

Fig. 8

From: SIRT1 mediates obesity- and nutrient-dependent perturbation of pubertal timing by epigenetically controlling Kiss1 expression

Fig. 8

Kiss1 expression is repressed by an SIRT1-EED silencing complex. a SIRT1 and EED repress KISS1 promoter activity as measured by gene reporter assays performed in Neuro2A cells. The bar histograms represent the mean ± SEM. *p < 0.05; **p < 0.01 (one-way ANOVA followed by the Student−Newman−Keuls; n = 4 biological replicates per group). b SIRT1 and EED coimmunoprecipitate as determined in 293T cells transfected with SIRT1-HA and EED-V5 expression vectors; reciprocal pull-down assays are shown. c Sirt1 mRNA in rat hypothalamic R22 cells infected with a control (C) lentiviral construct expressing green fluorescent protein (LV-GFP) or SIRT1. d CHIP assay showing association of SIRT1-HA to the Kiss1 promoter. e Kiss1 mRNA in C and SIRT1 overexpressing hypothalamic R22 cells. f Detection of H3K9Ac, H3K4me3 and H3K27me3 in C or SIRT1 overexpressing R22 cells. g Recruitment of EED to the Kiss1 promoter in C and SIRT1 overexpressing R22 cells. The bar histograms represent the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 vs. C group (two-sided Student’s t test; n = 4 per group)

Back to article page