Fig. 3

Energy homoeostasis parameters in WT and Npffr2^−/− mice under chow and HFD conditions. a–d Time-course of energy expenditure (a, b) and physical activity (c, d) in WT (open circle) and Npffr2^−/− (filled circle) mice of both genders on chow. e–h Time-course of energy expenditure (e, f) and physical activity (g, h) in WT and Npffr2^−/− mice of both genders on HFD. i–n Energy expenditure (i, j), physical activity (k, l) and respiratory exchange ratio (m, n) averaged over 24 h in WT and Npffr2^−/− mice fed on chow or HFD. Male chow: WT n = 8, Npffr2^−/− n = 14; male HFD: WT n = 9, Npffr2^−/− n = 7. Female chow: WT n = 14, Npffr2^−/− n = 17; female HFD: WT n = 8, Npffr2^−/− n = 11. Data are mean ± SEM. One-way ANOVA (i–n) or repeated measures (a–h) were used to determine genotype or diet effects among groups. ^∗p < 0.05 vs. WT of the same diet. ^#p < 0.05 vs. chow group of the same genotype