Fig. 8

Altered diet-induced obesity and energy homoeostasis in mice with conditional NPFFR2 deletion in NPY neurons under chow and HFD conditions. a, d Growth curve of male conditional KO and control mice on chow and HFD. Chow: control n = 14, conditional KO n = 13. HFD: control n = 14, conditional KO n = 10. b, c Weights of individual and summed white adipose tissue depots in male conditional KO and control mice under chow and HFD conditions. WATi, WATe, WATm and WATr represent inguinal, epididymal, mesenteric and retroperitoneal white adipose tissue, respectively. N values are as in a, d. e Energy intake in male conditional KO and control mice under chow and HFD conditions. Chow: control n = 16, conditional KO n = 10. HFD: control n = 14, conditional KO n = 10. f, i, l Energy expenditure time-course and averages over the light, dark and 24 h periods in male conditional KO and control mice under chow and HFD conditions. Chow: control n = 8, conditional KO n = 9. HFD: control n = 14, conditional KO n = 9. g, j, m Physical activity time-course and averages over the light, dark and 24 h periods in male conditional KO and control mice under chow and HFD conditions. N values are as in f, i, l. h, k, n Respiratory exchange ratio time-course and averages over the light, dark and 24 h periods in male conditional KO and control mice under chow and HFD conditions. N values are as in f, i, l. Gene deletion was induced by tamoxifen in male Npy^creER2/+,Npffr2^lox/lox mice at 6 weeks of age (Conditional KO). Male Npy^creER2/+,Npffr2^lox/lox mice without tamoxifen and Npy^creER2/+,Npffr2^lox/+ mice treated with tamoxifen were used as controls. Data are mean ± SEM. One-way ANOVA b, c, e, l, m, n or repeated measures a, d, f–h, i–k was used to determine difference among groups. ^∗∗p < 0.05 as indicated by bar