Fig. 1
From: Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR
2D and 3D 1H ssNMR of BamAP4P5 and BamA TM in proteoliposomes. a 1H detected 2D NH spectrum of BamA TM in liposomes. Close-ups of b and c show correlations for the POTRA domains. d Representative 1H line-width extracted from a. e Representative 2D slices from a 3D CαNH experiment that show correlations for residues I284 and T400 which are within 1 ppm in all three frequencies from the solution NMR assignments. f Sequential assignment of the E285/Q286 pair based on solution assignments and ssNMR 3D CαNH and CONH spectra. g From a CαNH experiment in a region of spectral overlap, A363/F395, or e.g. A318 that does not agree with the ssNMR data. Inset in a is a representation of the protein constructs used. In all panels red crosses are ssNMR assignments and in black are solution NMR assignments (BMRB 19928). In g, the 15N resonance frequencies of the relevant 2D planes are indicated
