Fig. 3

BamAP4P5–BamCDE interface in bilayers. a Close up of representative correlations from the 2D ¹³C–¹³C spectra of AVLTI labeled BamAP4P5 in liposomes, in the absence and presence of BamCDE (blue and red spectra, respectively). Crosses are assignments of the labeled residues; dashed boxes indicate correlations with chemical shift perturbations above 0.7 ppm. b BamAP4P5–BamCDE interaction interface as described by ssNMR data in lipid bilayers. Residues as sticks are labeled (i.e. AVLTI), those in gray were not used for analysis. Residues in red show a chemical shift perturbation, those that in yellow indicate an increase in intensity and those that in green do not experience any effect upon complex formation. The zoom-in shows in more detail the interaction interface. Indicated are the structural elements mentioned in the text and residue I668 of EL6 (spheres). c BamA–BamCDE interface as described by all BAM complex crystal structures (PDB 5D0O, 5D0Q, 5AYW, and 5EKQ). Contacts with the BamCDE complex, in red, were found using PDBePISA²⁹ on these structures. BamD and BamE are shown as semi-transparent surfaces in b, c, as well as a schematic representation of the liposome bilayer and detergent micelle are present to aid visualization