Fig. 2
Preferential expression of Dusp10 in Tpath2 cells. a–d Resting Tpath2 cells and ILC2s were used in RNA-sequencing. Cells isolated from spleens of memory Th2 mice (as shown in Supplementary Fig. 1a). After cultivation with IL-7, IL-25 plus IL-33 for 5 days, cells were rested with IL-7 alone for 24 h and analyzed by RNA-sequencing. a Venn diagram shows the genes preferentially expressed in Tpath2 cells and ILC2s. Immune response-related genes (GO0006955) preferentially expressed by unstimulated Tpath2 cells (Red) or unstimulated ILC2s (Blue) (>5-fold difference), or genes shared by the two (Purple) (<1.2-fold difference) are shown. Transcripts expressed above 10 fragments per kilobase of exon per million reads mapped (FPKM) are shown. b Gene Ontology analysis of genes that are expressed more highly in Tpath2 cells as compared to ILC2s. Graph shows folds change of indicated GO terms based on sub-categories including biological process (BP), cellular component (CC), and molecular function (MF) in Tpath2 cells to ILC2s (False discovery rate < 0.05). X-axis shows the number of fold-change (p < 0.02, fold change of at least 10, BP biological process, CC cellular component, MF molecular function). Transcripts expressed above 10 FPKM in Tpath2 cells were used. c A heatmap of genes distinctly expressed between Tpath2 cells and ILC2s in the category of MAP kinase phosphatase activity as in b. d Quantitative RT-PCR analysis of the kinases and phosphatases related to IL-33–ST2 signaling pathway in Tpath2 cells and ILC2s. Two independent experiments were performed and showed similar results (a–c). More than three independent experiments were performed and showed similar results (d) (**p < 0.01). Three technical replicates were performed with quantitative RT-PCR (d)
