Fig. 1
A functional Tim17–Pam18 fusion causes reorganization of the TIM23 complex. a Schematic representation of the Tim17–Pam18 fusion. N, N terminus; C, C terminus; IMS, intermembrane space; IM inner membrane. b Tenfold serial dilutions of wild-type and indicated Tim17–Pam18 strains were spotted on YPD and YPG plates and grown at indicated temperatures. c Western blot analysis of cell lysates from wild-type and Tim17–Pam18 strains. Asterisk, unspecific signal of antibody. Tim17–Pam18 protein was detected using Pam18 antibody. d Western blot analysis of steady-state protein using isolated mitochondria (5, 10, and 20 µg). e Digitonin-solubilized mitochondria were subjected to anti-Tim23 immunoisolation and samples were analyzed by western blotting. Total, 10%; elution, 100%
