Table 2 Nucleotide sequences of primers used for quantitative qPCR detection. “F” indicates the forward primer and “R” the reverse primer. Gapdh, rplp0, and Emg1 were used for normalization
From: Curvotaxis directs cell migration through cell-scale curvature landscapes
Gene name | Sequences (5′→3′) | Genbank accession number | qPCR product size (nucleotides) |
|---|---|---|---|
Gapdh | F-TTCAACAGCAACTCCCACTC R-ATGTAGGCCATGAGGTCCAC | NM_008084.3 | 137 |
Rplp0 | F-AACGGCAGCATTTATAACCC R-CGATCTGCAGACACACACTG | NM_007475.5 | 106 |
Emg1 | F-ACGGCCCTCAGAAGCTATT R-TCACTACTGGGCACCAACTC | NM_013536.2 | 133 |
RhoA | F-CTCATAGTCTTCAGCAAGGACC R-GGCGGTCATAATCTTCCTGTC | NM_001313961 | 147 |
Rac1 | F-TTATGACAGATTGCGTCCCC R-TGTCATAATCCTCTTGCCCTG | NM_009007 | 143 |
Cdc42 | F-CATGTCTCCTGATATCCTACACAAC R-TGTCATAATCCTCTTGCCCTG | NM_009861 | 93 |
Sun1 | F-CTGGGACGGTTCACCTATGA R-CCCGGAGCTCTACTATCTGGA | NM_024451 | 97 |
LmnA | F-CCGCTCTCATCAACTCCACT R-TCTCCATCCTCGTCGTCATC | NM_001111102 | 100 |
