Fig. 5

mRNA-expressed palivizumab prevents RSV infection in vivo. Mice lungs were transfected with 100 μg of naked aPali mRNA. Saline, negative, and irrelevant mRNA controls are indicated. 24 h later, mice were infected with RSV L19. At 4 days post infection, mice were sacrificed and lungs were excised. A total of 10 mice were used per group, out of which, 8 were processed for mRNA, protein, and virion extraction, with 2 being prepared for cryosectioning. aPali HC and Flu aVHH controls, along with a RSV only group, were performed in a separate experiment. a Virion content of mice lung homogenate supernatants was assayed by plaque assay. Error bars represent standard deviation. Asterisk indicates p < 0.05 (t-test, one-tailed). Hash indicates p = 0.07. b Mean RSV F copy number was determined by qRT-PCR. Data was normalized by saline control. Error bars represent standard deviation. Asterisk indicates p < 0.05 (t-test, one-tailed). c Cryosections were stained with panRSV antibody (green). Scale bar represents 100 μm. d Quantification of mean volume of RSV signal per frame from microscopy images in part c. Error bars represent 95% confidence intervals. Asterisks indicate p < 0.01 (Kruskal-Wallis, Dunn’s multiple comparisons). e For thresholded comparison, images with less than 400 μm³ signal were excluded. Error bars represent 95% confidence intervals. Asterisks indicate p < 0.0001 (Kruskal–Wallis, Dunn’s multiple comparisons)