Fig. 4
Ambient glutamate and glutamate transporters play critical roles in GNE-8324 potentiation of NMDAR EPSCs in inhibitory neurons. a Reduced NMDAR responses to ambient glutamate after CPG treatment. Sample traces showing reduced d-APV-induced holding current changes in inhibitory neurons treated with CPG. Scale bars, 10 pA/500 ms. d-APV-induced decrease in holding current was significantly smaller in CPG-treated neurons than in vehicle-treated neurons. *P < 0.05, unpaired t test. b CPG treatment eliminated GNE-8324 potentiation in the inhibitory neurons. Sample traces of NMDAR EPSCs before and after GNE-8324 application in the inhibitory neurons incubated with CPG. Scale bars, 20 pA/100 ms. Compared to the robust potentiation in vehicle-treated neurons, CPG-treated neurons showed no significant GNE-8324 potentiation of NMDAR EPSCs. *P < 0.05, unpaired t test. c GNE-8324 potentiation was eliminated in the inhibitory neurons from mice injected with ceftriaxone (Cef). Sample traces of NMDAR EPSCs before and after GNE-8324 application from an inhibitory neuron in Cef-injected mouse. Scale bars, 10 pA/100 ms. Effect of GNE-8324 in inhibitory neurons from Cef-injected and Veh-injected mice. **P < 0.01, unpaired t test. Error bars represent SEM
