Fig. 4
From: Targeting of NLRP3 inflammasome with gene editing for the amelioration of inflammatory diseases
Detection of Cas9 expression and NLRP3 knockout efficiency in macrophages of mice injected with CLANmCas9/gNLRP3. a, b Immunoblot analysis of Cas9 protein expression (a) or the T7E1 assay of indels introduced into the NLRP3 locus (b) in peritoneal macrophages of mice injected with CLANmCas9/gNLRP3 at 12 or 24 h post-injection. n = 5 per group. c Representative sequences of indels in the NLRP3 locus, see also Supplementary Fig. 10. d Immunoblot analysis of NLRP3 and other inflammasome protein expression in peritoneal macrophages of mice injected with CLANmCas9/gNLRP3 at 72 h post-injection. n = 5 per group. The data are representative of two independent experiments (a, d) or are shown as the means ± SEM of n = 3 (b)
