Fig. 2
From: An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
Significantly reduced leaky expression from basal promoter. a Relative luciferase expression levels at different developmental stages of pVALIUM20-luciferase and pNP-luciferase flies without the Gal4 driver (n = 5, mean ± s.d.). b Relative luciferase expression levels in different tissues of pVALIUM20-luciferase and pNP-luciferase flies at larval stage without the Gal4 driver (n = 3, mean ± s.d.). c Immunostaining of polytene chromosome from pVALIUM20-Hp1a and pNP-Hp1a without the induction of Gal4. DNA was visualized with DAPI and HP1a was stained with mouse monoclonal anti-HP1a (HP1a C1A9). Dotted circles indicate chromocenter. Scale bars, 20 μm. d Quantification of the relative HP1a intensity at chromocenter of polytene chromosome in pVALIUM20-Hp1a or pNP-Hp1a without the Gal4 driver (n = 10, mean ± s.d.). Data are evaluated with one-tailed Student’s t-test. e Relative expression of HP1a in control, pVALIUM20-Hp1a or pNP-Hp1a salivary gland without the driving of Gal4 (n = 3, mean ± s.d.). Data are evaluated using one-tailed Student’s t-test. f The heterozygous percentage of pVALIUM20 system based transgenic lines and pNP system based transgenic lines
